MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT CCR6 RECEPTOR

Product Information

Catalog Number:
C1014a

Lot Number:
C1014a-110609

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human CCR6 DNA (GenBank Accession Number NM_004367) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: CCR6 is a receptor for macrophage inflammatory protein (MIP)-3α (CCL20). It is expressed in immature dendritic cells as well as in B lymphocytes and memory T cells. In gene-targeted mice lacking CCR6, myeloid dendritic cells are either absent or underrepresented in the subepithelial dome compared with wild-type mice. CCR6 and its ligands contribute to the normal homeostatic regulation of extrathymic T cell development in the intestine.

Application: Functional assays

Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Dose-dependent stimulation of pERK level upon treatment with ligand, monitored with FlexStation. Figure 3. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Baba et al. (1997) Identification of CCR6, the specific receptor for a novel lymphocytedirected CC chemokine LARC. J Biol Chem 272:14893-14898.

Cook et al. (2000) CCR6 mediates dendritic cell localization, lymphocyte homeostasis, and immune responses in mucosal tissue. Immunity 12:495-503.

Lugering et al. (2003) Lymphoid precursors in intestinal cryptopatches express CCR6 and undergo dysregulated development in the absence of CCR6. J Immunol 171:2208-2215.

FOR RESEARCH USE ONLY.
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