MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CCR5 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length human CCR5 cDNA (GenBank Accession Number NM_000579) with flag tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: CCR5 (chemokine (C-C motif) receptor 5) is a receptor for C-C type chemokines MIP-1-α, MIP-1-ß and CCL5. CCR5 plays a role in the control of granulocytic lineage proliferation or differentiation. It also acts as co-receptor with CD4 for primary non-syncytium-inducing strains (macrophage-tropic) of HIV-1 virus.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin- stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Deng et al. (1996) Identification of a major co-receptor for primary isolates of HIV-1. Nature 381:661-666.
Oppermann (2004) Chemokine receptor CCR5: insights into structure, function, and regulation. Cell Signal 16:1201-1210.