MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CCR2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CCR2 cDNA (GenBank accession number NM_000647.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: CCR2 (C-C chemokine receptor type 2 or monocyte chemoattractant protein 1 receptor) is a receptor for the MCP-1, MCP-3 and MCP-4 chemokines. It is also an alternative coreceptor with CD4 for HIV-1 infection. CCR2 has been widely considered as a potential therapeutic target for autoimmune disease and inflammatory disorders of the lung, particularly rheumatoid arthritis, and various CCR2 blocking agents have been developed, some of which might have therapeutic values.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Quinones et al. (2005) The complex role of the chemokine receptor CCR2 in collagen-induced arthritis: implications for therapeutic targeting of CCR2 in rheumatoid arthritis. J Mol Med 83:672-681.
Rose et al. (2003) Significant involvement of CCL2 (MCP-1) in inflammatory disorders of the lung. Microcirculation 10:273-288.