MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CXCR5 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CXCR5 cDNA (GenBank Accession Number NM_001716.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable for a minimum of 2 months in continuous culture
Background: CXCR5 (CXC-chemokine receptor 5, or Burkitt lymphoma receptor 1) is a receptor for CXCL13 and expresses in mature B-cells, a subpopulation of T-cells and Burkitt lymphoma cells. CXCR5 exerts possibly a regulatory function in Burkitt lymphoma lymphomagenesis and/or B-cell differentiation. It is a potential candidate for cell-cell interaction and activation of mature B-lymphocytes in lymphatic tissues.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, monitored with FLIPR and measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (MSCA01). No Gαqi5 transfection. Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM cAMP 1.0 No Wash Assay Kit (MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Dobner et al. (1992) Differentiation-specific expression of a novel G protein coupled receptor from Burkitt’s lymphoma. Eur J Immunol 22:2795-2799.
Campbell et al. (2003) Chemokines in the systemic organization of immunity. Immunol Rev 195:58-71.