MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT CXCR5 RECEPTOR

Product Information

Catalog Number:
C1005a

Lot Number:
C1005a-120616

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human CXCR5 cDNA (GenBank Accession Number NM_001716.2) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable for a minimum of 2 months in continuous culture

Data Sheet

Background: CXCR5 (CXC-chemokine receptor 5, or Burkitt lymphoma receptor 1) is a receptor for CXCL13 and expresses in mature B-cells, a subpopulation of T-cells and Burkitt lymphoma cells. CXCR5 exerts possibly a regulatory function in Burkitt lymphoma lymphomagenesis and/or B-cell differentiation. It is a potential candidate for cell-cell interaction and activation of mature B-lymphocytes in lymphatic tissues.

Application: Functional assays

Figure 1. Dose-dependent calcium flux upon treatment with ligand, monitored with FLIPR and measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (MSCA01). No Gαqi5 transfection. Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM cAMP 1.0 No Wash Assay Kit (MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Dobner et al. (1992) Differentiation-specific expression of a novel G protein coupled receptor from Burkitt’s lymphoma. Eur J Immunol 22:2795-2799.

Campbell et al. (2003) Chemokines in the systemic organization of immunity. Immunol Rev 195:58-71.

FOR RESEARCH USE ONLY.
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