MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT CXCR5 RECEPTOR

Product Information

Catalog Number:
C1005

Lot Number:
C1005-060909

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human CXCR5 cDNA (GenBank Accession Number NM_001716.2) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: CXCR5 (CXC-chemokine receptor 5, or Burkitt lymphoma receptor 1) is a receptor for CXCL13 and expresses in mature B-cells, a subpopulation of T-cells and Burkitt lymphoma cells. CXCR5 exerts possibly a regulatory function in Burkitt lymphoma lymphomagenesis and/or B-cell differentiation. It is a potential candidate for cell-cell interaction and activation of mature B-lymphocytes in lymphatic tissues.

Application: Functional assays

Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Dobner et al. (1992) Differentiation-specific expression of a novel G protein coupled receptor from Burkitt’s lymphoma. Eur J Immunol 22:2795-2799.

Campbell et al. (2003) Chemokines in the systemic organization of immunity. Immunol Rev 195:58-71.

FOR RESEARCH USE ONLY.
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