MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CXCR5 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CXCR5 cDNA (GenBank Accession Number NM_001716.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 µg/mL puromycin
Stable in culture for a minimum of 1 month
Background: CXCR5 (CXC-chemokine receptor 5, or Burkitt lymphoma receptor 1) is a receptor for CXCL13 and expresses in mature B-cells, a subpopulation of T-cells and Burkitt lymphoma cells. CXCR5 exerts possibly a regulatory function in Burkitt lymphoma lymphomagenesis and/or B-cell differentiation. It is a potential candidate for cell-cell interaction and activation of mature B-lymphocytes in lymphatic tissues.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Dobner et al. (1992) Differentiation-specific expression of a novel G protein coupled receptor from Burkitt’s lymphoma. Eur J Immunol 22:2795-2799.
Campbell et al. (2003) Chemokines in the systemic organization of immunity. Immunol Rev 195:58-71.