MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT CXCR3 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CXCR3 cDNA (GenBank Accession Number: NM_001504) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable in culture for minimum of two months
Background: CXCR3 (chemokine receptor 3) is a receptor for SCYB9/MIG, SCYB10/INP10 and SCYB11/ITAC. The chemokines that target CXCR3 in concert with Th1 cytokine appear to play a beneficial role to atopic disorders including a range of conditions such as allergic asthma, -rhinitis, -conjunctivitis, -dermatitis, food and drug allergies and anaphylaxis.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor Expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
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Arimilli, S., W. Ferlin, et al. (2000). “Chemokines in autoimmune diseases.” Immunol Rev 177: 43-51.