MULTISCREEN™ STABLE CELL LINES

HUMAN RECOMBINANT CXCR3 RECEPTOR

Product Information

Catalog Number:
C1003

Lot Number:
C1003-031506

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human CXCR3 cDNA (GenBank Accession Number: NM_001504) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: CXCR3 (chemokine receptor 3) is a receptor for SCYB9/MIG, SCYB10/INP10 and SCYB11/ITAC. The chemokines that target CXCR3 in concert with Th1 cytokine appear to play a beneficial role to atopic disorders including a range of conditions such as allergic asthma, -rhinitis, -conjunctivitis, -dermatitis, food and drug allergies and anaphylaxis.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor Expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Loetscher, M., B. Gerber, et al. (1996). “Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes.” J Exp Med 184(3): 963-9.

Arimilli, S., W. Ferlin, et al. (2000). “Chemokines in autoimmune diseases.” Immunol Rev 177: 43-51.

FOR RESEARCH USE ONLY.
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