MULTISCREEN™ MEMBRANE PREPARATION
RAT RECOMBINANT EP2 RECEPTOR
1 vial (16.12mg/mL), 1, 5, or 10mg
Packaging Buffer: 20mM Gly-Gly, 1 mM MgCl2, 25mM Sucrose (pH 7.2)
Expression vector containing full-length rat EP2 cDNA (GenBank Accession Number NM_031088.1) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving. Avoid repeated freeze-thaw
Background: The human prostaglandin E2 (PGE2) receptor EP2 (PTGER2) is abundantly expressed in various tissues including the corneal epithelium of the eye, spinal cord, forebrain, articular cartilage, and kidney. EP2 plays important roles in bronchodilation, dilation of arterioles and venules, blood pressure regulation, smooth muscle relaxation, and bone formation. Modification of PGE2-EP2 receptor signaling may provide a new therapeutic strategy for renal regulation and blood pressure illnesses, as well as bone disease such as osteoarthritis.
Figure 1: Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells thick line: receptor-expressing cells.
Morath et al. (1999) Immunolocalization of the four prostaglandin E2 receptor proteins EP1,
EP2, EP3, and EP4 in human kidney. J Am Soc Nephrol 10:1851-1860.
Zhang et al. (2000) Characterization of murine vasopressor and vasodepressor
prostaglandin E(2) receptors. Hypertension 35:1129-1134.
Li X et al. (2009) Prostaglandin E(2) and its cognate EP receptors control human adult articular cartilage homeostasis and are linked to the pathophysiology of osteoarthritis. Arthritis Rheum 60:513-523.