MULTISCREEN™ MEMBRANE PREPARATION

HUMAN RECOMBINANT β2 ADRENERGIC RECEPTOR

Product Information

Catalog Number:
MC1438-1a

Lot Number:
MC1438-1a-08032012

Quantity:
8.0 mg/ml, 1mg

Packaging Buffer:
20 mM Gly-Gly, 1 mM MgCl2, 250 mM Sucrose

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human ADRB2 cDNA (GenBank Accession Number NM_000024) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Data Sheet

Background: Norepinephrine is implicated in a wide range of physiological processes through activation of nine different G-protein-coupled receptors (α1a, α1b, α1d, α2a, α2b, α2c, ß1, ß2, ß3). The human ß2-adrenergic receptor was the first 7- transmembrane receptor for a hormone or neurotransmitter to have its crystal structure solved. It has been suggested that the β2-adrenoceptor may form homodimers as well as oligomers with other receptors. The β2-adrenoceptor mediates the actions of catecholamines in multiple tissues. They are responsible for relaxation of vascular, uterine, and airway smooth muscle, and are involved in metabolic and endocrine functions.

Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Kobilka et al. (1987) cDNA for the human beta 2-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor. Proc Natl Acad Sci USA 84:46-50.

Frielle et al. (1989) Properties of the beta 1- and beta 2-adrenergic receptor subtypes revealed by molecular cloning. Clin Chem 35:721-725.

FOR RESEARCH USE ONLY.
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