MULTISCREEN™ MEMBRANE PREPARATION

HUMAN RECOMBINANT α2A RECEPTOR

Product Information

Catalog Number:
MC1434-1a

Lot Number:
MC1434-1a-06192012

Quantity:
1 vial (15.6 mg/ml)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human α2a cDNA (GenBank Accession Number: NM_000681) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Data Sheet

Background: Adrenergic α2a receptor belongs to the group of nine adrenoceptors that mediate the biological actions of adrenaline and noradrenaline. The α2a receptor is expressed in the brain, spleen, kidney, heart and gastrointestinal tract. It is centrally predominant and exerts a tonic sympathoinhibitory function, whereas peripherally it has a vasoconstrictive effect. The α2a receptor is also involved in sedation, digestive functions and analgesia.

Application: [35S]GTPγS Binding Assay

Figure 1. Dose-dependent [35S]GTPγS Binding to α2a membrane upon treatment with agonist using homogenous proximity assay method. Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Knaus et al (2007) Alpha2-adrenoceptor subtypes–unexpected functions for receptors and ligands derived from gene-targeted mouse models. Neurochem Int 51:277-281.
Philipp et al (2002) Physiological significance of alpha(2)-adrenergic receptor subtype diversity: one receptor is not enough. Am J Physiol Regul Integr Comp Physiol 283:R287-R295.

FOR RESEARCH USE ONLY.
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