MULTISCREEN™ MEMBRANE PREPARATION

HUMAN RECOMBINANT DP2 RECEPTOR

Product Information

Catalog Number:
MH1105a

Lot Number:
MH1105a-07202012

Quantity:
1 vial (15.6mg/ml)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human OPRL1 cDNA (GenBank Accession Number NM_000913.3) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen

Data Sheet

Background: The chemoattractant-homologous receptor expressed on TH2 cells (CRTH2, also known as DP2 and GPR44) serves as a receptor for prostaglandin D2 (PGD2) and has been reported to mediate PGD2-dependent migration of eosinophils. DP2 mediates the pro-inflammatory effects of PGD2 generated in allergic inflammation. 11-Dehydro- thromboxane B2 (11-dehydro-TXB2) was found to be a full agonist of the DP2 receptor and hence might cause the receptor activation in cellular contexts where PGD-synthase is not present. Antagonism of the 11-dehydro-TXB2/DP2 axis may be of therapeutic relevance.

Application: Functional assays

Figure 1. Dose-dependent [35S]GTPγS Binding to DP2 membrane upon treatment with agonist using homogenous proximity assay method. Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Nagata et al. (1999) CRTH2, an orphan receptor of T-helper-2-cells, is expressed on basophils and eosinophils and responds to mast cell-derived factor(s). FEBS Lett 459:195- 199.

Bohm et al. (2004) 11-Dehydro-thromboxane B2, a stable thromboxane metabolite, is a full agonist of chemoattractant receptor-homologous molecule expressed on TH2 cells (CRTH2) in human eosinophils and basophils. J Biol Chem 279:7663-7670.

FOR RESEARCH USE ONLY.
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