MULTISCREEN™ MEMBRANE PREPARATION
MOUSE RECOMBINANT S1P4 RECEPTOR
1 vial (2 x 106) frozen cells
Cellbanker 2 (Amsbio 11891)
Expression vector containing full-length mouse S1P2 cDNA (Genbank Accession Number: NM_010333.4) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin
Background: S1P4 receptor or Endothelial Differentiation Gene-6 (EDG-6) is receptor for sphingosine-1-phosphate (S1P). S1P is a bioactive lipid produced from the metabolism of sphingomyelin. It is an important constituent of serum and regulates cell growth, survival, migration, differentiation and gene expression. Unlike other members of S1P receptor, which are widely expressed, S1P4 exhibits lymphoid tissue-specific expression. EDG-6 has been implicated in regulation of cell shape and motility.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Clemens et al. (2004) Synthesis of benzimidazole based analogues of sphingosine- 1-phosphate: discovery of potent, subtype-selective S1P4 receptor agonists. Bioorg Med Chem Lett 14:4903-4906.
Takuwa et al. (2001) Subtype-specific, differential activities of the EDG family receptors for sphingosine-1-phosphate, a novel lysophospholipid mediator. Mol Cell Endocrinol 177:3-11.