MULTISCREEN™ MEMBRANE PREPARATION
HUMAN RECOMBINANT SMO RECEPTOR
1 vial (18.8mg/ml, 10mg) membrane
Expression vector containing full-length human SMO cDNA (GenBank Accession Number: NM_005631.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Background: The human smoothened homolog SMO strongly resembles that of the frizzled family of proteins, the receptors of the Wnt signaling pathway, which share several features with the Hedgehog pathway. Most often SMO functions during embryonic development, in processes such as digit patterning in the chick limb bud and left–right asymmetry of vertebrate embryos. In addition, SMO function is fundamental for the maintenance of tissue homeostasis in adults, and deregulated SMO signaling is implicated in tumorogenesis.
Application: Radioligand Binding Assay
Figure 1. Saturation binding assay curve: Specific binding obtained with SMO membrane with [3H]cyclopamine. Figure 2. Competition binding assay curve: Dose dependent competition of SAG for [3H]cyclopamine 5nM. Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Ruiz-Gómez et al. (2007) The cell biology of Smo signaling and its relationships with GPCRs. Biochim Biophys Acta 1768:901-912.