MULTISCREEN™ MEMBRANE PREPARATION
HUMAN RECOMBINANT A2B RECEPTOR
20 mM Gly-Gly, 1 mM MgCl2, 25 mM Sucrose (pH 7.2)
Expression vector containing full-length human ADORA2B cDNA (GenBank accession number NM_000676.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Background: A2B is a receptor for adenosine. A2B receptor is upregulated during intestinal inflammation and mediates key events such as chloride, IL-6 and fibronectin secretion in intestinal epithelial cells. A2B receptor antagonists may have important clinical value in the treatment of inflammatory diseases, such as asthma and chronic obstructive pulmonary disease (COPD), as well as inflammatory bowel disease.
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Hasko et al. (2008) Adenosine receptors: therapeutic aspects for inflammatory and immune diseases. Nat Rev Drug Discov 7:759-770.
Linden et al. (1999) Characterization of human A (2B) adenosine receptors: radioligand binding, wester blotting, and coupling to G(q) in human embryonic kidney 293 cells and HMC-1 mast cells. Mol Pharmacol 56:705-713.