MULTISCREEN™ MEMBRANE PREPARATION
HUMAN RECOMBINANT μ OPIOID RECEPTOR
1 vial (2.5 mg/ml)
Expression vector containing full-length human OPRM1 cDNA (GenBank Accession Number: NM_000914.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Background: μ opioid receptor (MOR) is a G protein-coupled receptor for β- endorphin. The receptor activation inhibits neurotransmitter release by reducing calcium currents and increasing potassium conductance. MOR mediates positive reinforcement following direct (morphine) or indirect (alcohol, cannabinoids, nicotine) activation. MOR plays a genetic role in the control of gut inflammation. MOR-deficient mice are highly susceptible to colon inflammation, with a 50% mortality rate occurring 3 days after administration of TNBS that induces inflammation. MOR agonists regulate cytokine production and T cell proliferation and might be new therapeutic molecules in inflammatory bowel disease.
Application: [35S]GTPγS Binding Assay
Figure 1. Dose-dependent [35S]GTPγS Binding to OPRM1 membrane upon treatment with agonist using homogenous proximity assay method. Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Chen et al. (1993) Molecular cloning and functional expression of a mu-opioid receptor from rat brain. Mol Pharmacol 44:8-12.
Contet et al. (2004) Mu opioid receptor: a gateway to drug addiction. Curr Opin Neurobiol 14:370-378.
Philippe et al. (2003) Anti-inflammatory properties of the mu opioid receptor support its use in the treatment of colon inflammation. J Clin Invest 111:1329-1338.