MULTISCREEN™ MEMBRANE PREPARATION
HUMAN RECOMBINANT CT RECEPTOR
1 vial (4.4 mg/mL, 1mg)
20mM Gly-Gly, 1mM MgCl2, 25mM Sucrose (pH 7.2)
Full-length Human CALCR cDNA (GenBank Accession Number NM_001742) with FLAG-tag sequence at the N-terminus
Store at -80°C. Avoid repeated freeze-thaw.
Background: Calcitonin receptor (CT or CALCR) is a receptor for calcitonin. In the presence of the receptor activity modifying proteins (RAMP1, 2 or 3), it acts as a receptor for amylin. CT is expressed in brain, bone, stomach, intestine, kidney and testes. Data from transgenic mice support a major role for calcitonin and its receptor in the formation and resorption aspects of bone metabolism under physiological conditions.
Application: Functional assays
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Gorn et al. (1992) Cloning, characterization, and expression of a human calcitonin receptor from an ovarian carcinoma cell line. J Clin Invest 90:1726-1735.
Taboulet et al. (1998) Calcitonin receptor polymorphism is associated with a decreased fracture risk in post-menopausal women. Hum Mol Genet 7:2129-2133.