MULTISCREEN™ MEMBRANE PREPARATION

HUMAN RECOMBINANT IP1 RECEPTOR

Product Information

Catalog Number:
MC1206-1

Lot Number:
MC1206-1-031314

Quantity:
11.7 mg/ml, 0.5 mg

Packaging Buffer:
20 mM Gly-Gly, 1 mM MgCl2, 25 mM Sucrose (pH 7.2)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human PTGIR cDNA (GenBank Accession Number: NM_000960) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Data Sheet

Background: The human prostaglandin I2 receptor IP1 (or PTGIR) mediates the actions of prostaglandin I2 (PGI2 or prostacyclin), which is a labile metabolite of arachidonic acid produced in concert with the bis-enoic prostaglandins via the cyclooxygenase pathway. PGI2 plays a major physiologic role as a strong mediator of vasodilation and inhibitor or platelet activation, and is an antithrombotic agent in vivo and mediates inflammation and pain. PGI2 derived from COX2 plays a critical role in regulating the release of rennin and consequently in renovascular hypertension.

Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Boie et al. (1994) Cloning and expression of a cDNA for the human prostanoid IP receptor. J Biol Chem 269:12173-12178.

Coleman et al. (1994) VIII International union of pharmacology classification of prostanoid receptors: properties, distribution, and structure of the receptors and their subtypes. Pharm Rev 46:205-229.

FOR RESEARCH USE ONLY.
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