MULTISCREEN™ MEMBRANE PREPARATION

HUMAN RECOMBINANT MGLUR7 RECEPTOR

Product Information

Catalog Number:
MC1194

Lot Number:
MC1194-12282011

Quantity:
11.2 mg/ml, 1mg

Packaging Buffer:
20 mM Gly-Gly, 1 mM MgCl2, 250 mM Sucrose

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human GRM7 cDNA (GenBank Accession Number NM_000844) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Data Sheet

Background: The metabotropic glutamate receptors (mGluRs) are divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacological properties. Group II and group III mGluRs are linked to inhibition of the cyclic AMP cascade, but differ in their agonist selectivity. mGluR7 is a member of Group III. Immunohistochemistry and in situ hybridization showed that mGluR7 expresses in various sites in the nervous system.

Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Wu et al. (1998) Group III human metabotropic glutamate receptors 4, 7, and 8: molecular cloning, functional expression, and comparison of pharmacological properties in RGT cells. Brain Res Mol Brain Res 53:88-97.

Makoff et al. (1996) Human metabotropic glutamate receptor type: molecular cloning and mRNA distribution in the CNS. Brain Res Mol Brain Res 40:165-170.

Muto et al.(2007) Structures of the extracellular regions of the group II/III metabotropic glutamate receptors. Proc Natl Acad Sci USA 104:3759-3764.

FOR RESEARCH USE ONLY.
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