MULTISCREEN™ MEMBRANE PREPARATION
HUMAN RECOMBINANT MGLUR6 RECEPTOR
9.6mg/ml, 0.5 mg
20 mM Gly-Gly, 1 mM MgCl2, 250 mM Sucrose
Expression vector containing full-length human GRM6 cDNA (NM_000843.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Background: The metabotropic glutamate receptors (mGluRs) are divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacological properties. Group II and group III mGluRs are linked to inhibition of the cyclic AMP cascade, but differ in their agonist selectivity. mGluR6 is a member of Group III. It mainly expresses in on-bipolar cells in retina.
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Laurie et al. (1997) Cloning, distribution and functional expression of the human mGlu6 metrbottropic glutamate receptor. Neuropharmacology 36:145-152.
Dryja et al. (2005) Night blindness and abnormal cone electroretinogram on responses in patients with mutations in the GRM6 gene encoding mGluR6. Proc Natl Acad Sci USA 102:4884–4889.