MULTISCREEN™ MEMBRANE PREPARATION

HUMAN RECOMBINANT OX2 RECEPTOR

Product Information

Catalog Number:
MC1034-1

Lot Number:
03/17/2010

Quantity:
13 vials (18 mg/mL, 1 mg)

Packaging Buffer:
20 mM Tris-HCl (pH7.5), 2 mM EDTA, protease inhibitor cocktail

Host cell:
CHO-K1

Transfection:
Full-length Human HCRTR2 cDNA (GenBank Accession Number NM_001526.2) with FLAG-tag sequence at the N-terminus

Recommended Storage:
Store at – 80°C. Avoid repeated freeze-thaw.

Data Sheet

Background: Orexins, or hypocretins, produced by a small group of neurons in lateral hypothalamus, are involved in many physiological functions such as feeding, energy metabolism, sleep, arousal, reward, substance abuse, stress, as well as sympathetic and cardiovascular functions. OX2 is one of the two receptors for the orexins. The receptor is expressed in the brain regions such as the hypothalamus, hippocampus and thalamus. Recent studies have indicated that orexins may also be useful in the treatment of schizophrenia and other psychiatric disorders.

Figure 1. Histogram showing receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Deutch and Bubser (2007) The orexins/hypocretins and schizophrenia. Schizophr Bull 33:1277-1283.

Smart et al. (1999) Characterization of recombinant human orexin receptor pharmacology in a Chinese hamster ovary cell-line using FLIPR. Br J Pharmacol 128:1-3.

FOR RESEARCH USE ONLY.
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