MULTISCREEN™ MEMBRANE PREPARATION

HUMAN RECOMBINANT CCR9 RECEPTOR

Product Information

Catalog Number:
MC1017

Lot Number:
03162012

Quantity:
1 vial (10.3mg/ml)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human CCR9 cDNA (GenBank Accession Number: NM_006641) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Data Sheet

Background: CCR9 (C-C chemokine receptor type 9) is a thymus-specific receptor for chemokine CCL25 (SCYA25 or TECK). It is an alternative co-receptor with CD4 for HIV-1 infection. Activation of the receptor may lead to potent cFLIP(L)- independent resistance to cycloheximide-induced apoptosis and modest resistance to Fas-mediated apoptosis possibly via activation of multiple signaling components involving Akt and glycogen synthase kinase 3β. Thus, dissection of signaling components involved in the CCR9-mediated antiapoptosis could be a framework for cell survival mechanisms and may provide options for therapeutic interventions.

Application: [35S]GTPγS Binding Assay

Figure 1. Dose-dependent [35S]GTPγS Binding to CCR9 membrane upon treatment with agonist using homogenous proximity assay method. Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Choe et al. (1998) The orphan seven-transmembrane receptor apj supports the entry of primary T-cell-line-tropic and dualtropic human immunodeficiency virus type 1. J Virol 72:6113-6118.

Youn et al. (2002) Role of the CC chemokine receptor 9/TECK interaction in apoptosis. Apoptosis 7:271-276.

FOR RESEARCH USE ONLY.
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