MULTISCREEN™ MEMBRANE PREPARATION
HUMAN RECOMBINANT CXCR6 RECEPTOR
1 vial (15.6 mg/ml), 1mg
20 mM Gly-Gly, 1 mM MgCl2, 25 mM Sucrose (pH 7.2)
Full-length Human CXCR6 cDNA (GenBank Accession Number NM_006564) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving, avoid freeze- thaw
Background: CXCR6 (CXC-chemokine receptor type 6) is a receptor for CXC chemokine CXCL16. CXCR6 is expressed by naive CD8 T cells, natural killer T cells, a subset of memory CD4 T cells and plasma cells. CXCR6 and its ligand play an important role in the trafficking of the cells expressing them. It is demonstrated that CXCL16+ macrophages in synovial fluid of rheumatoid arthritis patients lead to recruitment of CXCR6+ memory T cells, thereby contributing to the inflammatory cascade associated with rheumatoid arthritis pathology. CXCR6 is also used as a coreceptor by simian immunodeficiency viruses (SIVs) and by strains of HIV-2 and M- tropic HIV-1.
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Deng et al. (1997) Expression cloning of new receptors used by simian and human immunodeficiency viruses. Nature 388:296-300.
Blaak et al. (2005) CCR5, GPR15, and CXCR6 are major coreceptors of human immunodeficiency virus type 2 variants isolated from individuals with and without plasma viremia. J Virol 79:1686-1700.