MULTISCREEN™ MEMBRANE PREPARATION

HUMAN RECOMBINANT CXCR4 RECEPTOR

Product Information

Catalog Number:
MC1004-1

Lot Number:
07/15/11

Quantity:
1 vial (11.8 mg/mL,1 mg)

Packaging Buffer:
20 mM Gly-Gly, 1 mM MgCl2, protease 25 mM Sucrose (pH 7.2)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human CXCR4 cDNA (GenBank Accession Number NM_003467) with FLAG tag sequence at N-terminus

Recommended Storage:
Store at -80°C. Avoid repeated freeze-thaw.

Data Sheet

Background: CXCR4 is a receptor for the C-X-C chemokine SDF-1 (Stromal Cell- Derived Factor 1). It is involved in haematopoiesis and cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, cerebellar development and survival of hippocampal-neuron of central nerve system. CXCR4 also acts as a primary receptor for some HIV-2 isolates and as a co-receptor with CD4 for HIV-1 X4 viruses.

Figure 1. Histogram showing receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Hernandez et al. (2003) Mutations in the chemokine receptor gene CXCR4 are associated with WHIM syndrome, a combined immunodeficiency disease. Nat Genet 34:70-74.

Juarez et al. (2004) Chemokines and their receptors as therapeutic targets: the role of the SDF-1/CXCR4 axis. Curr Pharm Des 10:1245-1259.

FOR RESEARCH USE ONLY.
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