MULTISCREEN™ MEMBRANE PREPARATION
HUMAN RECOMBINANT CXCR4 RECEPTOR
1 vial (11.8 mg/mL,1 mg)
20 mM Gly-Gly, 1 mM MgCl2, protease 25 mM Sucrose (pH 7.2)
Expression vector containing full-length human CXCR4 cDNA (GenBank Accession Number NM_003467) with FLAG tag sequence at N-terminus
Store at -80°C. Avoid repeated freeze-thaw.
Background: CXCR4 is a receptor for the C-X-C chemokine SDF-1 (Stromal Cell- Derived Factor 1). It is involved in haematopoiesis and cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, cerebellar development and survival of hippocampal-neuron of central nerve system. CXCR4 also acts as a primary receptor for some HIV-2 isolates and as a co-receptor with CD4 for HIV-1 X4 viruses.
Figure 1. Histogram showing receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Hernandez et al. (2003) Mutations in the chemokine receptor gene CXCR4 are associated with WHIM syndrome, a combined immunodeficiency disease. Nat Genet 34:70-74.
Juarez et al. (2004) Chemokines and their receptors as therapeutic targets: the role of the SDF-1/CXCR4 axis. Curr Pharm Des 10:1245-1259.