MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT M4 RECEPTOR
1 vial (4 x 106) frozen cells
Cellbanker 2 (Amsbio 11891)
Expression vector containing full-length human CHRM4 cDNA (GenBank accession number NM_000741.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
DMEM/F12, 10% FBS, 10 μg/mL puromycin
1-2 days after thawing
Background: The human muscarinic M4 receptor is a 479-amino acid, 7-transmembrane protein. In heart muscle, M4 receptors are coupled to specific K+ currents that regulate cardiac muscle contractions. They can also be found in tissues of the central nervous system and bladder.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Eglen (2006) Muscarinic receptor subtypes in neuronal and non-neuronal cholinergic function. Auton Atacoid Pharmcol 26:219-233.
Yeh et al. (2007) Atrial tachycardia induces remodeling of muscarinic receptors and their coupled potassium currents in canine left atrial and pulmonary vein cardiomyocytes. Br J Pharmacol 152:981-982.