MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT α2C RECEPTOR

Product Information

Catalog Number:
DC1436-1

Lot Number:
DC1436-1-072419

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Cell Banker 2

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human ADRA2C cDNA (GenBank Accession Number AY455666) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS

Data Sheet

Background: The human adrenergic α2c receptor is a 458-amino acid, 7- transmembrane protein. It belongs to the group of nine adrenoceptors that mediate the biological actions of the endogenous catecholamines adrenaline and noradrenaline. All three alpha2-receptor subtypes may operate as presynaptic inhibitory feedback receptors to control the release of neurotransmitters. The α2c receptor is of particular importance as it appears to regulate venous vasoconstriction.

Application: Functional assays

Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).

References:

Gyires et al. (2009) alpha(2)-Adrenoceptor subtypes-mediated physiological, pharmacological actions. Neurochem Int 55:447-453.

Knaus et al (2007) Alpha2-adrenoceptor subtypes–unexpected functions for receptors and ligands derived from gene-targeted mouse models. Neurochem Int 51:277-281.

FOR RESEARCH USE ONLY.
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