MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT α2C RECEPTOR
1 vial (4 x 106) frozen cells
Cell Banker 2
Expression vector containing full-length human ADRA2C cDNA (GenBank Accession Number AY455666) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS
Background: The human adrenergic α2c receptor is a 458-amino acid, 7- transmembrane protein. It belongs to the group of nine adrenoceptors that mediate the biological actions of the endogenous catecholamines adrenaline and noradrenaline. All three alpha2-receptor subtypes may operate as presynaptic inhibitory feedback receptors to control the release of neurotransmitters. The α2c receptor is of particular importance as it appears to regulate venous vasoconstriction.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
Gyires et al. (2009) alpha(2)-Adrenoceptor subtypes-mediated physiological, pharmacological actions. Neurochem Int 55:447-453.
Knaus et al (2007) Alpha2-adrenoceptor subtypes–unexpected functions for receptors and ligands derived from gene-targeted mouse models. Neurochem Int 51:277-281.