MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT α1a ADRENERGIC RECEPTOR

Product Information

Catalog Number:
DC1431-1a

Lot Number:
DC1431-1a-021016

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human ADRA1A cDNA (GenBank accession number NM_000680.2) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS

Stability:
1-2 days after thawing

Data Sheet

Background: Norepinephrine is implicated in a wide range of physiological processes through activation of nine different G-protein-coupled receptors (α1a, α1b, α1d, α2a, α2b, α2c, ß1, ß2, ß3). The α1a-adrenoceptor is highly expressed in human vasculature. As with the other α1-ARs, α1a is used by the sympathetic nervous system to regulate systemic arterial blood pressure and blood flow. The α1-ARs also play a major role in cardiac and vascular smooth muscle cells. The knockout mouse models lacking the α1a-adrenergic receptors have highlighted the potential implications of this receptor subtype in variety of functions including the regulation of renal artery contractions, smooth muscle contractions, and vasoconstriction.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Chalothorn et al. (2002) Differences in the cellular localization and agonist-mediated internalization properties of the α1-adrenoreceptor subtypes. Mol Pharmacol 61(5):1008-1016.

Weinberg et al. (1994) Cloning, expression and characterization of human alpha adrenergic receptors alpha 1a, alpha 1b and alpha 1c. Biochem. Biophys. Res. Commun. 201:1296-1304.

Hague et al. (2003) a1-Adrenergic receptor subtypes: non-identical triplets with different dancing partners? Life Sciences 74:411-418.

FOR RESEARCH USE ONLY.
All rights reserved. No part of this document may be reproduced in any form without prior permission in writing.

26219 Eden Landing Road Hayward, CA 94545 | (510) 887-0817 | info@multispaninc.com