MULTISCREEN™ DIVISION-ARRESTED CELL LINE

HUMAN RECOMBINANT A1 RECEPTOR

Product Information

Catalog Number:
DC1427-1

Lot Number:
DC1427-1-032316

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human ADORA1 cDNA (GenBank Accession Number NM_000674) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM-F12, 10% FBS

Stability:
Stable for 1 – 2 days after thawing

Data Sheet

Background: Adenosine A1 receptor (ADORA1 or RDC7) is a G protein- coupled receptor for adenosine. RT-PCR detected A1 expression in the brain, colon, jejunum, ileum and kidney. A1 functions in sleep-wake regulation and the fertilization process.

Application: Functional assays

Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Ethier and Madison (2006) Adenosine A1 receptors mediate mobilization of calcium in human bronchial smooth muscle cells. Am J Respir Cell Mol Biol 35:496-502.

FOR RESEARCH USE ONLY.
All rights reserved. No part of this document may be reproduced in any form without prior permission in writing.

26219 Eden Landing Road Hayward, CA 94545 | (510) 887-0817 | info@multispaninc.com