MULTISCREEN™ DIVISION-ARRESTED CELL LINE

HUMAN RECOMBINANT PTH1 RECEPTOR

Product Information

Catalog Number:
DC1301-1

Lot Number:
DC1301-1-053020

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Cell Banker 2

Host cell:
HEK293T

Transfection:
Full-length Human PTH1R cDNA (GenBank Accession Number NM_000316.2) with FLAG-tag sequence at the N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS

Data Sheet

Background: Parathyroid hormone receptor (PTH1R) functions as a receptor for parathyroid hormone (PTH) and parathyroid hormone-related protein (PTHrP). It is expressed in high levels in bone and kidney and mediates mineral ion homeostasis as well as the paracrine actions of PTHrP, which is important in the process of endochondral bone formation. Defects in PTH1R can cause chondrodysplasia and enchondromatosis. The development of novel PTH receptor ligands could prove to be valuable in the treatment of diseases such as osteoporosis and hypercalcemia.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Mannstadt et al. (1999) Receptors for PTH and PTHrP: their biological importance and functional properties. Am. J. Physiol. 277 (5 Pt 2): F665-75.

Langub et al. (2001) Parathyroid hormone/parathyroid hormone-related peptide type 1 receptor in human bone. J. Bone. Miner. Res. 16: 448-456.

FOR RESEARCH USE ONLY.
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