MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT CASR RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CASR cDNA (GenBank accession number NM_000388) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable for 1-2 days after thawing
Background: CASR is a calcium-sensing receptor and plays an important role in regulating PTH secretion. It is expressed in many different tissues, such as parathyroid cells, pituitary cells, kidney, fibroblasts, keratinocytes and human colon epithelial cells. CASR is a potential therapeutic target for the treatment of many diseases, including hyperparathyroidism and osteoporosis. Mutations in the CASR gene can result in gain or loss of receptor function. Familial Hypocalciuric Hypercalcemia (FHH) and Neonatal Severe Primary Hyperparathyroidism (NSHPT) have been associated with loss of CASR function, while Autosomal Dominant Hypocalcemia (ADH) and Bartter syndrome type V have been associated with gain of CASR function.
Application: Functional assays
Figure Legend. Dose-dependent accumulation of intracellular IP1 upon treatment with ligand, measured with IP-one Tb kit (Cisbio 62IPAPEC).
D’Souza-Li (2006) The calcium-sensing receptor and related diseases. Arq Bras Endocrinol Metabol 50:628-639.
Romoli et al. (1999) Expression of calcium-sensing receptor and characterization of intracellular signaling in human pituitary adenomas. J Clin Endocrinol Metab 84:2848-2853.