MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT BB1 RECEPTOR
1 vial (4 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human NMBR cDNA (GenBank Accession Number NM_002511.1) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
1-2 days after thaw
Background: The human BB1 receptor (or Neuromedin B receptor NMBR) is a receptor for neuromedin-B (NMB), which is a mammalian bombesin-like peptide distributed widely in the central nervous system. The BB1 pathway is involved in the regulation of a wide variety of behaviors, such as spontaneous activity, feeding and anxiety-related behavior. A study using BB1-deficient mice suggested that dysfunction in the BB1 pathway may constitute one of the risk factors of stress vulnerability.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Benya et al. (1995) Expression and characterization of cloned human bombesin receptors. Mol Pharmacol 47:10-20.
Moody et al. (2000) Nonpeptide neuromedin B receptor antagonists inhibit the proliferation of C6 cells. Eur J Pharmacol 409:133-142.
Yamada et al. (2002) Restraint stress impaired maternal behavior in female mice lacking the neuromedin B receptor (NMB-R) gene. Neurosci Lett 330:163-166.