MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT FP RECEPTOR
1 vial (4 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human PTGFR cDNA (GenBank accession number NM_000959) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable for 1-2 days after thawing
Background: Prostaglandin F (2-alpha) is involved in a number of physiologic processes. It serves as a potent luteolytic agent in many species, has been implicated as a modulator of intraocular pressure, smooth muscle contraction in the uterus and elsewhere. Its effects on cells are mediated through specific interaction with the prostaglandin F receptor (FP or PTGFR). Knockout mice lacking the FP receptor are unable to deliver normal fetuses at term due to a lack of response to oxytocin. The mice also failed to show the decline in serum progesterone expected to precede parturition.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Abramovitz et al. (1994) Cloning and expression of a cDNA for the human prostanoid FP receptor. J Biol Chem 269(4):2632-6.
Kim et al. (2012) Prostaglandin FP receptor inhibitor reduces ischemic brain damage and neurotoxicity. Neurobiol Dis 48(1):58-65.