MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT P2Y6 RECEPTOR
1 vial (4 x 106) frozen cells
Cellbanker 2 (Amsbio)
Expression vector containing full-length human P2Y6 cDNA (GenBank Accession Number NM_004154) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Background: The human P2Y6 receptor is a member of the G protein-coupled P2 receptor family that responds specifically to the extracellular nucleotide uridine diphosphate (UDP). The distribution of the P2Y6 receptor is widespread including heart, blood vessels and brain. P2Y6 receptors are significantly upregulated in T cells infiltrating regions of inflamed bowel and are involved in monocytic release of IL-8 in response to UDP or lipopolysaccharide (LPS). The immune effect of UDP/P2Y6 signaling on the production of proinflammatory cytokines has been shown to be selective and dependent on cell types.
Application: Functional assays
Figure 1: Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Communi et al. (1996) Cloning, functional expression and tissue distribution of the human P2Y6 receptor. Biochem Biophys Res Commun 222:303-308.
Warny et al. (2001) P2Y(6) nucleotide receptor mediates monocyte interleukin-8 production in response to UDP or lipopolysaccharide. J Biol Chem 276:26051-26056.