MULTISCREEN™ DIVISION ARRESTED CELL LINE

MOUSE RECOMBINANT MGLUR4 RECEPTOR

Product Information

Catalog Number:
DHGm1191-1b

Lot Number:
05/31/13

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO Gqi5

Transfection:
Expression vector containing full-length mouse GRM4 cDNA (GenBank Accession Number BC072635) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F-12 with glutaGRO (Corning 10-103-CV), 10% FBS (dialyzed)

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. The metabotropic glutamate receptors (mGluRs), which are G protein-coupled receptors, have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group II and group III mGluRs are linked to the inhibition of the cyclic AMP cascade, but differ in their agonist selectivity. Group III agonists include L-2-amino-4- phosphonobutyrate (L-AP4) and L-serine-O-phosphate (Wu et al., 1998).

Application: Functional assays

Figure Legend. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Wu et al. (1998) Group III human metabotropic glutamate receptors 4, 7 and 8: molecular cloning, functional expression, and comparison of pharmacological properties in RGT cells. Mol Brain Res 53:88 97.

FOR RESEARCH USE ONLY.
All rights reserved. No part of this document may be reproduced in any form without prior permission in writing.

26219 Eden Landing Road Hayward, CA 94545 | (510) 887-0817 | info@multispaninc.com