MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT GPR43 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human FFAR2 cDNA (GenBank Accession Number NM_005306) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS
Stable for 1-2 days after thawing
Background: GPR43, or FFAR2, encodes a deduced 330-amino acid protein with 7 transmembrane domains. GPR43 is expressed by enteroendocrine L cells containing peptide YY in the human large intestine. The receptor binding of short-chain fatty acids potentially provides a molecular link between diet, gastrointestinal bacterial metabolism, and immune and inflammatory responses.
Application: Ca++ assays
Figure legend: Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Sawzdargo et al. (1997) Cluster of four novel human G protein-coupled receptor genes occurring in close proximity to CD22 gene on chromosome 19q13.1. Biochem Biophys Res Commun 239:543-547.