MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT CCK2 RECEPTOR

Product Information

Catalog Number:
DH1039

Lot Number:
DH1039-020714

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human CCKBR cDNA (GenBank Accession Number NM_176875.2) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS

Stability:
Stable for 1 – 2 days after thawing

Data Sheet

Background: Cholecystokinin B receptor (CCKBR/CCK2R) is a 447-amino acid 7 transmembrane protein. The cholecystokinin (CCK) family of peptides and their receptors are widely distributed throughout the central nervous system and gastrointestinal tract. CCKBR is a type B (gastrin) receptor that has high affinity for both sulfated and nonsulfated CCK analogs found principally in the CNS and select areas of the gastrointestinal tract. Type B receptors regulate anxiety, arousal, neuroleptic activity and opiate-induced analgesia. Outside of the CNS they regulate gastric acid secretion and may play a role in gastrointestinal motility and growth of normal and neoplastic gastrointestinal tissue. Northern hybridization shows CCKBR expressed in the stomach, pancreas, brain and gallbladder.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, monitored with FLIPR.

References:

Beinborn et al. (1993) A single amino acid of the cholecystokinin-B/gastrin receptor determines specificity for non-peptide antagonists. Nature 362:348-350.

Pisegna et al. (1992) Molecular cloning of the human brain and gastric cholecystokinin receptor: structure, functional expression and chromosomal localization. Biochem Biophys Res Commun 189:296-303.

FOR RESEARCH USE ONLY.
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