MULTISCREEN™ DIVISION ARRESTED CELL LINE

RAT RECOMBINANT GPR120 RECEPTOR

Product Information

Catalog Number:
DCr1294

Lot Number:
DCr1294-08032012

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
full-length rat GPR120 cDNA (GenBank accession Number NM_001047088.1) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: GPR120 is a G protein-coupled receptor for the long-chain free fatty acids. GPR120 mediated calcium mobilization, Erk1/Erk2 activation and GLP1 secretion. Unsaturated long-chain FFAs had a dose-dependent stimulatory effect, and α-linolenic acid was the most potent. GPR120 and GLP1 colocalized in human colonic intraepithelial neuroendocrine cells, and GPR120 may mediate dietary FFA- stimulated GLP1 secretion.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, monitored with FlexStation. Figure 2. Receptor Expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Hirasawa et al. (2005) Free fatty acids regulate gut incretin glucagon-like peptide-1 secretion through GPR120. Nature Med 11:90-94.

Tanaka et al. (2008) Cloning and characterization of the rat free fatty acid receptor GPR120: in vivo effect of the natural ligand on GLP-1 secretion and proliferation of pancreatic beta cells. Naunyn Schmiedebergs Arch Pharmacol 377:515-522.

FOR RESEARCH USE ONLY.
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