MULTISCREEN™ DIVISION ARRESTED CELL LINE
RAT RECOMBINANT GIP RECEPTOR
1 vial (4 x 106) frozen cells
Cellbanker 2 (Amsbio 11891)
Expression vector containing full-length rat GIPR cDNA (GenBank accession number P43219) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
1-2 days after thawing
Background: GIP (gastric inhibitory polypeptide) is released from the gastrointestinal tract, stimulates insulin secretion from pancreatic beta-cells, and plays a crucial role in the regulation of insulin secretion. Its receptor GIPR is expressed in the pancreas, stomach, small intestine, adipose tissue, adrenal cortex, pituitary, heart, testis, endothelial cells, bone, trachea, spleen, thymus, lung, kidney, thyroid, and several regions in the CNS. GIPR may have therapeutic potential in the treatment of type 2 diabetes and obesity.
Application: Functional assays
Figure 1. Dose-dependent stimulation of intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
Irwin et al. (2009) Therapeutic potential for GIP receptor agonists and antagonists. Best Pract Res Clin Endocrinol Metab 23:499-512.
Yamada et al. (1995) Human gastric ingibitory polypeptide receptor: cloning of the gene (GIPR) and cDNA. Genomics 29:773-776.