MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT GPR35 RECEPTOR
1 vial (4 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human GPR35 cDNA (GenBank Accession Number NM_005301) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS
stable for 1-2 days after thawing
Background: GPR35 is an orphan receptor and expressed in various tissues including stomach, gastrointestinal tissues, and several types of immune cells. Up- regulation of GPR35 has been found in human mast cells upon stimulation with IgE antibodies, human macrophages treated with the environmental contaminant polycyclic aromatic hydrocarbon benzo[a]pyrene, failing heart cells, and gastric cancer cells. Known agonists of the orphan receptor GPR35 are kynurenic acid, zaprinast, 5-nitro-2-(3-phenylproplyamino) benzoic acid, and lysophosphatidic acids.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Wang JH et al. (2006) Kynurenic Acid as a Ligand for Orphan G Protein-coupled Receptor GPR35. J Biol Chem 281:22021-22028.
MacKenzie1, AE, et al. (2011) GPR35 as a novel therapeutic target. Frontiers in Endocrinology, 2:1-10.
Zhao PW, et al. (2010) Targeting of the Orphan Receptor GPR35 by Pamoic Acid: A Potent Activator of Extracellular Signal-Regulated Kinase and beta-Arrestin2 with Antinociceptive Activity. Mol Pharm 78:560–568.