MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT CXCR4 RECEPTOR

Product Information

Catalog Number:
DCG1004

Lot Number:
DCG1004-042716

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T Gαqi5

Transfection:
Expression vector containing full-length human CXCR4 cDNA (GenBank Accession Number NM_003467) with FLAG tag sequence at N- terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS

Stability:
Stable for 1 – 2 days after thawing

Data Sheet

Background: CXCR4 is a receptor for the C-X-C chemokine SDF-1 (Stromal Cell-Derived Factor 1). It is involved in haematopoiesis and cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, cerebellar development and survival of hippocampal-neuron of central nerve system. CXCR4 also acts as a primary receptor for some HIV-2 isolates and as a co-receptor with CD4 for HIV-1 X4 viruses.

Application: Functional assays

Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Hernandez et al. (2003) Mutations in the chemokine receptor gene CXCR4 are associated with WHIM syndrome, a combined immunodeficiency disease. Nat Genet 34:70-74.

Juarez et al. (2004) Chemokines and their receptors as therapeutic targets: the role of the SDF- 1/CXCR4 axis. Curr Pharm Des 10:1245-1259.

FOR RESEARCH USE ONLY.
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