MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT CXCR3 RECEPTOR

Product Information

Catalog Number:
DCG1003

Lot Number:
11/14/12

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T Gαqi5

Transfection:
Expression vector containing full-length human CXCR3 cDNA (GenBank Accession Number: NM_001504) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: CXCR3 (chemokine receptor 3) is a receptor for SCYB9/MIG, SCYB10/INP10 and SCYB11/ITAC. The chemokines that target CXCR3 in concert with Th1 cytokine appear to play a beneficial role to atopic disorders including a range of conditions such as allergic asthma, -rhinitis, -conjunctivitis, -dermatitis, food and drug allergies and anaphylaxis.

Application: Functional assays

Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Loetscher, M., B. Gerber, et al. (1996). “Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes.” J Exp Med 184(3): 963-9.

Arimilli, S., W. Ferlin, et al. (2000). “Chemokines in autoimmune diseases.” Immunol Rev 177: 43-51.

FOR RESEARCH USE ONLY.
All rights reserved. No part of this document may be reproduced in any form without prior permission in writing.

26219 Eden Landing Road Hayward, CA 94545 | (510) 887-0817 | info@multispaninc.com