MULTISCREEN™ DIVISION-ARRESTED CELL LINE

HUMAN RECOMBINANT 5-HT4 RECEPTOR

Product Information

Catalog Number:
DC1330

Lot Number:
DC1330-013118

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Cellbanker 2 (AMSBio, Cat# 11891)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human HTR4 cDNA (GenBank accession number NM_000870.4) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: The human serotonin receptor 5-HT4 is a G protein-coupled receptor. 5-HT4 receptors can be found in the central nervous system and in peripheral tissues such as the alimentary tract, urinary bladder, heart and adrenal gland. 5-HT4 ligands may prove to be therapeutic in the treatment of disorders such as cardiac arrhythmias, irritable bowel syndrome and urinary incontinence.

Application: Functional assays

Figure Legend: Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.

References:

Talley (2003) Evaluation of drug treatment in irritable bowel syndrome. Br J Clin Pharmacol 56:362-369.

Van den Wyngaert et al. (1997) Cloning and expression of a human serotonin 5-HT4 receptor DNA. J Neurochem 69:1810-1819.

FOR RESEARCH USE ONLY.
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