MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT 5-HT2C RECEPTOR

Product Information

Catalog Number:
DC1326-1

Lot Number:
DC1326-1-08/19/09

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1

Transfection:
Full-length Human HTR2C cDNA (GenBank Accession Number NM_000868) with FLAG-tag sequence at the N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: 5-HT2C (5-hydroxytryptamine receptor 2C) is a receptor for serotonin. It is expressed in the brain and spinal cord, especially the choroid plexus. 5-HT2C receptor agonists may have important clinical value in the treatment of mental and eating disorders, such as depression, panic anxiety, OCD, bulimia and obesity.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Martin et al. (1998) 5-HT2C receptor agonists: Pharmacological characteristics and therapeutic potential. J Pharmacol Exp Ther 286:913-924.

Ni and Miledi (1997) Blockage of 5HT2C serotonin receptors by fluoxetine (Prozac). Proc Natl Acad Sci USA 94:2036-2040.

Porter et al. (1999) Functional characterization of agonists at recombinant human 5- HT2A, 5-HT2B, and 5-HT2C receptors in CHO-K1 cells. Br J Pharmacol 128:13-20.

FOR RESEARCH USE ONLY.
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