Product Information
Catalog Number:
DC1319a
Lot Number:
DC1319a-111014
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium:
Sigma Freezing Medium (C-6164)
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human HTR1A cDNA (GenBank accession number NM_000524.2) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stability:
Stable for 1-2 days after thawing
Data Sheet
Background: The human serotonin receptor 5-HT1A is a G protein-coupled receptor. 5-HT1A receptors can be found mainly in the cerebral cortex, hippocampus, septum, amygdala, and raphe nucelus, with lesser amounts in the basal ganglia and thalamus. 5-HT1A ligands may prove to be therapeutic in the treatment of various disorders such as depression, anxiety, and schizophrenia.
Application: Functional assays
Figure Legend. Dose-dependent stimulation of pERK level upon treatment with ligand, monitored with FlexStation (Cisbio 64AERPEG).
References:
Hamon et al. (1990) The main features of central 5-HT1 receptors. Neuropsychopharmacology 3:349-360.
Savitz et al. (2009) 5-HT(1A) receptor function in major depressive disorder. Prog Neurobiol 88:17-31.
Stam et al. (1992) Genomic organization, coding sequence and functional expression of human 5-HT2 and 5-HT1A receptor genes. Eur J Pharmacol 227:153-162.