MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT 5-HT1A RECEPTOR
1 vial (4 x 102) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human HTR1A cDNA (GenBank Accession Number NM_000524.2) with FLAG tag sequence at N-terminus
nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable 1-2 days after thawing
Background: The human serotonin receptor 5-HT1A is a G protein-coupled receptor. 5-HT1A receptors can be found mainly in the cerebral cortex, hippocampus, septum, amygdala, and raphe nucelus, with lesser amounts in the basal ganglia and thalamus. 5-HT1A ligands may prove to be therapeutic in the treatment of various disorders such as depression, anxiety, and schizophrenia.
Application: Functional assays
Figure 1. Dose-dependent stimulation of pERK level upon treatment with ligand, monitored with FlexStation. 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Hamon et al. (1990) The main features of central 5-HT1 receptors. Neuropsychopharmacology 3:349-360.
Savitz et al. (2009) 5-HT(1A) receptor function in major depressive disorder. Prog Neurobiol 88:17-31.
Stam et al. (1992) Genomic organization, coding sequence and functional expression of human 5-HT2 and 5-HT1A receptor genes. Eur J Pharmacol 227:153-162.