MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT OT RECEPTOR
1 vial (4 x 106) frozen cells
Cell Banker 2 (Amsbio 11891)
Full-length Human OXTR cDNA (GenBank Accession Number NM_000619.3) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DME/F12, 10% FBS, 10 µg/mL puromycin
1 – 2 days after thawing
Background: Oxytocin (OT) is a member of the neurohypophyseal hormone family. OT receptors are found in uterine smooth muscle, myoepithelial cells in the mammary gland, and in the pituitary. OT stimulates contraction of uterine smooth muscle during labor and stimulates milk secretion in response to suckling. It has been postulated that OT may also facilitate social and bonding behaviors related to the reproduction and care of offspring. Further studies of the OT receptor are essential in gaining a better understanding of the mechanism and in vivo regulation of uterine function.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Gimpl and Fahrenholz (2001) The oxytocin receptor system: structure, function, and regulation. Physiol Rev 81:629-683.
Ivell et al. (2001) The structure and regulation of the oxytocin receptor. Exp Physiol 86:289-296.
Shojo and Kaneko (2000) Characterization and expression of oxytocin and the oxytocin receptor. Mol Genet Metab 71:552-558.