MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT Y5 RECEPTOR

Product Information

Catalog Number:
DC1275a

Lot Number:
DC1275a-011118

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Cellbanker 2 (Amsbio 11891)

Host cell:
HEK293T

Transfection:
Full-length Human NPY5R cDNA (GenBank Accession NM_006174) with FLAG-tag sequence at the N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS

Stability:
Stable for 1 – 2 days after thawing

Data Sheet

Background: NPY5R encodes 456-amino acid protein with 7 putative transmembrane domains. It has shown to be expressed in the brain and hypothalamus. Y5 receptors’ pharmacological profile correlates it with peptides known to elicit a feeding response. It has been suggested to have effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system.

Application: Functional assays

Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).

References:

Gerald et al. (1996) A receptor subtype involved in neuropeptide-Y induced food intake. Nature 382:168-171.

Hu et al. (1996) Identification of a novel hypothalamic neuropeptide Y receptor associated with feeding behavior. J Biol Chem 217:26315-26319.

FOR RESEARCH USE ONLY.
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