HUMAN RECOMBINANT BLT2 RECEPTOR

MULTISCREEN™ DIVISION ARRESTED CELL LINE

Product Information

Catalog Number:
DC1272A

Lot Number:
DC1272A-100814

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human BLT2 cDNA (GenBank Accession Number NM_001164692.2) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: Leukotriene B4(LTB4)isapotentlipidmediatorofallergicandinflammatory reactions, as well as a modulator of immune responses. Two receptors for LTB4 have been described on human neutrophils. The high-affinity human leukocyte LTB4 receptor, BLT1 mediates aggregation, chemotaxis, chemokinesis, and increased adherence to surfaces, whereas the low-affinity receptor, BLT2 (or LTB4R2) mediates degranulation and increased oxidative metabolism. BLT2 is expressed ubiquitously with the highest expression in spleen and has a broader ligand specificity for various eicosanoids. Cells expressing BLT2 exhibited LTB4-induced chemotaxis, calcium mobilization, and inhibition of adenylyl cyclase. BLT2 provides a novel target for anti-inflammatory therapy and promises to expand our knowledge of LTB4 function.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Kamohara et al. (2000) Molecular cloning and characterization of another leukotriene B4 receptor. J Biol Chem 275:27000-27004.

Yokomizo et al. (2000) A second leukotriene B(4) receptor, BLT2. A new therapeutic target in inflammation and immunological disorders. J Exp Med 192:421-432.

Yoo et al. (2004) Role of the BLT2, a leukotriene B4 receptor, in Ras transformation. Oncogene 23:9259-9268.

FOR RESEARCH USE ONLY.
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