MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT CB2 RECEPTOR
1 vial (4 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CNR2 cDNA (GenBank Accession Number NM_001841) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS
1-2 days after thawing
Background: Cannabinoid receptor 2 (CB2 or CNR2) is involved in cannabinoid- induced central nerve system effects. It could be a receptor for anandamide. The receptor is a potential therapeutic target in the treatment of various disease conditions, such as pain, multiple sclerosis, vascular disease, Parkinson’s disease, and other central nerve system disorders.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
Mendizabal and Adler-Graschinsky (2003) Cannabinoid system as a potential target for drug development in the treatment of cardiovascular disease. Curr Vasc Pharmacol 1:301-313.
Gerard et al. (1990) Nucleotide sequence of a human cannabinoid receptor cDNA. Nucleic Acids Res 18:7142.