MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT CB2 RECEPTOR

Product Information

Catalog Number:
DC1230-1a

Lot Number:
DC1230-1a-101615

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human CNR2 cDNA (GenBank Accession Number NM_001841) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS

Stability:
1-2 days after thawing

Data Sheet

Background: Cannabinoid receptor 2 (CB2 or CNR2) is involved in cannabinoid- induced central nerve system effects. It could be a receptor for anandamide. The receptor is a potential therapeutic target in the treatment of various disease conditions, such as pain, multiple sclerosis, vascular disease, Parkinson’s disease, and other central nerve system disorders.

Application: Functional assays

Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).

References:

Mendizabal and Adler-Graschinsky (2003) Cannabinoid system as a potential target for drug development in the treatment of cardiovascular disease. Curr Vasc Pharmacol 1:301-313.

Gerard et al. (1990) Nucleotide sequence of a human cannabinoid receptor cDNA. Nucleic Acids Res 18:7142.

FOR RESEARCH USE ONLY.
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