MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT MGLUR8 RECEPTOR

Product Information

Catalog Number:
DC1195

Lot Number:
DC1195-02/11/11

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human mGluR8 cDNA (GenBank Accession Number NM_000845.1) with FLAG tag sequence at N-terminus.

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM with GlutaMAX (Gibco 10566), 10% FBS (dialyzed), 2 mM sodium pyruvate.

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: The metabotropic glutamate receptors (mGluRs) have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group II and group III mGluRs are linked to the inhibition of the cyclic AMP cascade, but differ in agonist selectivity. Group III agonists include L-2-amino- 4-phosphonobutyrate (L-AP4) and L-serine-O-phosphate. The human mGluR8 protein shares 67 to 70% sequence similarity with mGluR4 and mGluR7 and 99% amino acid identity with mouse mGluR8.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Scherer et al. (1996) Localization of two metabotropic glutamate receptor genes, GRM3 and GRM8, to human chromosome 7q. Genomics 31:230-233.

Scherer et al. (1997) The human metabotropic glutamate receptor 8 (GRM8) gene: a disproportionately large gene located at 7q31.3-q32.1. Genomics 44:232-236.

FOR RESEARCH USE ONLY.
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