MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT MGLUR7 RECEPTOR

Product Information

Catalog Number:
DC1194

Lot Number:
DC1194-072214

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human GRM7 cDNA (GenBank Accession Number NM_000844) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM with GlutaMAX (Gibco 10566), 10% FBS (dialyzed), 2 mM sodium pyruvate

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: The metabotropic glutamate receptors (mGluRs) are divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacological properties. Group II and group III mGluRs are linked to inhibition of the cyclic AMP cascade, but differ in their agonist selectivity. mGluR7 is a member of Group III. Immunohistochemistry and in situ hybridization showed that mGluR7 expresses in various sites in the nervous system.

Application: Functional assays

Figure 1. Dose-dependent response of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).

References:

Wu et al. (1998) Group III human metabotropic glutamate receptors 4, 7, and 8: molecular cloning, functional expression, and comparison of pharmacological properties in RGT cells. Brain Res Mol Brain Res 53:88-97.

Makoff et al. (1996) Human metabotropic glutamate receptor type: molecular cloning and mRNA distribution in the CNS. Brain Res Mol Brain Res 40:165-170.

Muto et al.(2007) Structures of the extracellular regions of the group II/III metabotropic glutamate receptors. Proc Natl Acad Sci USA 104:3759-3764.

FOR RESEARCH USE ONLY.
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